Table 2.
Center of mass distances between of residue 37 of the C-terminal of amylin, its mutants and the cross-seeded assemblies. The distances are measured between residue 37 of each strand and the adjacent successive one, excluding the less stable terminal chains 1 and 10.
| Y37/Y37, distance (Å) | WT oligomer | F15L oligomer | F23L oligomer | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Run 1 | Run 2 | Run 3 | Run 1 | Run 2 | Run 3 | Run 1 | Run2 | Run 3 | |
| Chains-2_3 | 11.14(4.0) | 7.14(2.8) | 5.16(0.3) | 5.18(0.3) | 6.22(1.5) | 5.28(0.5) | 5.25(0.4) | 5.44(0.7) | 10.68(4.9) |
| Chains-3_4 | 6.02(2.5) | 5.13(0.3) | 5.06(0.2) | 5.05(0.2) | 5.14(0.3) | 5.03(0.3) | 5.14(0.3) | 5.14(0.3) | 5.14(0.3) |
| Chains-4_5 | 6.92(1.8) | 5.04(0.2) | 5.02(0.2) | 5.00(0.2) | 4.99(0.2) | 5.00(0.2) | 5.00(0.2) | 5.02(0.2) | 5.02(0.3) |
| Chains-5_6 | 5.14(0.4) | 5.02(0.3) | 5.00(0.3) | 4.95(0.2) | 4.95(0.2) | 4.99(0.2) | 4.95(0.2) | 4.97(0.2) | 4.99(0.2) |
| Chains-6_7 | 5.06(0.2) | 5.05(0.3) | 5.40(0.8) | 4.99(0.2) | 4.97(0.2) | 4.92(0.2) | 4.98(0.2) | 4.99(0.2) | 5.02(0.2) |
| Chains-7_8 | 5.01(0.1) | 5.19(0.4) | 8.70(4.0) | 5.06(0.2) | 5.03(0.2) | 4.93(0.2) | 4.98(0.2) | 5.55(1.4) | 5.05(0.2) |
| Chains-8_9 | 5.05(0.3) | 6.45(2.7) | 8.74(6.4) | 5.76(0.8) | 5.17(0.4) | 5.00(0.3) | 4.99(0.2) | 9.93(4.2) | 5.06(0.2) |
| Mean± SD | 6.33 ± 2.2 | 5.57± 0.8 | 6.15± 1.8 | 5.14 ±0.3 | 5.21 ± 0.4 | 5.02± 0.1 | 5.04 ±0.1 | 5.86 ±1.8 | 5.85 ±2.1 |
| L37/L37, distance (Å) | Y37L oligomer | Res37/Res37, distance (Å) | WT-F15L hetero assembly oligomer | ||||
|---|---|---|---|---|---|---|---|
| Run 1 | Run 2 | Run3 | Run 1 | Run 2 | Run 3 | ||
| Chains-2_3 | 10.35(1.1) | 13.01(2.4) | 10.67(3.2) | Chains-2_3 (WT) | 5.22(0.5) | 16.21(3.4) | 5.10(0.2) |
| Chains-3_4 | 6.08(0.5) | 7.73(0.8) | 6.54(1.1) | Chains-3_4 (WT) | 5.09(0.3) | 5.45(1.1) | 4.99(0.2) |
| Chains-4_5 | 7.25(0.7) | 6.58(1.0) | 7.79(1.6) | Chains-4_5 (WT) | 5.06(0.3) | 5.32(0.5) | 4.92(0.2) |
| Chains-5_6 | 6.51(0.9) | 5.58(0.3) | 8.98(3.2) | Chains-5_6(interface) | 5.47(0.3) | 5.58(0.5) | 4.88(0.2) |
| Chains-6_7 | 13.20(6.5) | 7.50(0.8) | 10.77(3.7) | Chains-6_7 (F15L) | 6.19(1.2) | 7.64(1.5) | 4.88(0.2) |
| Chains-7_8 | 17.89(6.5) | 5.24(0.3) | 12.17(6.2) | Chains-7_8 (F15L) | 6.30(1.7) | 11.62(3.0) | 4.98(0.2) |
| Chains-8_9 | 9.86(2.9) | 5.61(0.4) | 14.09(5.4) | Chains-8_9(F15L) | 5.35(0.4) | 10.66(2.0) | 5.09(0.2) |
| Mean± SD | 10.16± 4.2 | 7.3 ± 2.7 | 10.1 ± 2.6 | Mean ± SD | 5.52 ± 0.5 | 8.92 ± 4.1 | 4.98 ± 0.1 |
| Res37/Res37, distance (Å) | WT-F23L hetero assembly oligomer | Res37/Res37, distance (Å) | WT-Y37L hetero assembly oligomer | ||||
| Run 1 | Run 2 | Run 3 | Run 1 | Run 2 | Run 3 | ||
| Chains-2_3 (WT) | 5.16(0.3) | 5.36(0.6) | 5.11(0.3) | Chains-2_3 (WT) | 7.37(1.5) | 16.48(0.2) | 16.89(4.4) |
| Chains-3_4 (WT) | 5.02(0.2) | 5.04(0.2) | 5.00(0.2) | Chains-3_4 (WT) | 9.44(1.5) | 9.15(0.2) | 11.62(4.0) |
| Chains-4_5 (WT) | 4.93(0.2) | 4.94(0.2) | 4.97(0.2) | Chains-4_5 (WT) | 6.74(1.4) | 9.92(0.4) | 6.39(0.7) |
| Chains-5_6 (interface) | 4.94(0.2) | 4.90(0.2) | 4.91(0.2) | Chains-5_6(interface) | 7.92(0.2) | 9.51(0.2) | 7.57(0.9) |
| Chains-6_7 (F23L) | 5.00(0.2) | 4.98(0.2) | 4.97(0.2) | Chains-6_7 (Y37L) | 5.40(0.5) | 5.34(0.4) | 5.19(0.4) |
| Chains-7_8 (F23L) | 5.05(0.3) | 5.03(0.2) | 5.06(0.2) | Chains-7_8 (Y37L) | 5.55(0.5) | 5.19(0.3) | 5.02(0.2) |
| Chains-8_9(F23L) | 5.42(1.0) | 4.97(0.3) | 5.04(0.3) | Chains-8_9(Y37L) | 7.76(1.6) | 5.17(0.4) | 5.03(0.2) |
| Mean± SD | 5.07± 0.2 | 5.03 ±0.2 | 5.01± 0.1 | Mean ±SD | 7.17 ±1.4 | 8.68 ±4.1 | 8.24 ±4.5 |