Table 4.
Results from the experimental validation of 5 sets of double mutations at the positions K279 and R437 for which constructed mutant viruses were rescued from plasmid transfected in Vero cells.
| MUTATION IN INFECTIOUS CLONE | GENESV PREDICTION | VIRUS RESCUED | PREDICTION VALIDATED? |
|---|---|---|---|
| (AAA)K-279-N(AAC) (AGA)R-437-E(GAA) |
Viable | Not Viable | No |
| (AAA)K-279-R(AGA) (AGA)R-437-E(GAA) |
Viable | Yes | Yes |
| (AAA)K-279-E(GAA) (AGA)R-437-K(AAA) |
Viable | Yes | Yes |
| (AAA)K-279-E(GAA) (AGA)R-437-Q(CAA) |
Viable | Yes | Yes |
| (AAA)K-279-E(GAA) (AGA)R-437-E(GAA) |
Not Viable | Not Viable | Yes |