Figure 4. Protection induced by recombinant E. muris P29 is associated with induction of a broad IgG isotype responses.
Serum IgG antibody responses specific to rP29 (A) and E. muris lysate antigen (B) in experimental groups before and on day 10 after E. muris challenge were determined by ELISA. (C) Serum immunoglobulin isotype responses specific to rP29 in mice immunized with rP29 before (rP29) and on day 10 after E. muris challenge (rP29/EM). (D) Serum immunoglobulin isotype responses specific to E. muris-lysate antigen in mice after primary (EM) and on day 10 after secondary E. muris infection (EM/EM). Data are representative of two independent experiments. (A & B) Each data point represents an individual immunized animal before E. muris challenge infection (open circles) or on day 10 after E. muris challenge (closed circles) and the horizontal solid bars represent the means. (C & D) Each bar represents the average of four immunized mice before E. muris challenge infection (open bars) or on day 10 after E. muris challenge (solid bars) and the error bars represent the standard deviation.