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. 2014 Jan 15;9(1):e81542. doi: 10.1371/journal.pone.0081542

Table 4. Inhibition effects (IC50) of different ligands in replacement assay of the 1, 8-ANS-bound SlSCPx-2.

Ligands IC50 (µM) RBA(%) Ligands IC50 (µM) RBA (%)
Arachidonate 18.12±0.83 277 Linoleic acid 143.00±0.99 35
Oleic acid 24.37±1.14 206 AeSCPI-1 160.90±5.03 31
Stearic acid 39.82±1.17 126 Ergosterol 167.60±0.34 30
Cholesterol 50.13±0.80 100 Stigmasterol 189.60±2.29 26
AeSCPI-2 55.03±0.66 91 Octanoate 217.90±1.53 23
Palmitic acid 59.05±1.29 85

IC50: the concentration at 50% inhibition and the data are derived from assays described in Fig. 4B. RBA: relative binding affinity. It was calculated by dividing the IC50 of cholesterol ( = 100%) by the IC50 of each chemical.