Table 4. Inhibition effects (IC50) of different ligands in replacement assay of the 1, 8-ANS-bound SlSCPx-2.
Ligands | IC50 (µM) | RBA(%) | Ligands | IC50 (µM) | RBA (%) |
Arachidonate | 18.12±0.83 | 277 | Linoleic acid | 143.00±0.99 | 35 |
Oleic acid | 24.37±1.14 | 206 | AeSCPI-1 | 160.90±5.03 | 31 |
Stearic acid | 39.82±1.17 | 126 | Ergosterol | 167.60±0.34 | 30 |
Cholesterol | 50.13±0.80 | 100 | Stigmasterol | 189.60±2.29 | 26 |
AeSCPI-2 | 55.03±0.66 | 91 | Octanoate | 217.90±1.53 | 23 |
Palmitic acid | 59.05±1.29 | 85 |
IC50: the concentration at 50% inhibition and the data are derived from assays described in Fig. 4B. RBA: relative binding affinity. It was calculated by dividing the IC50 of cholesterol ( = 100%) by the IC50 of each chemical.