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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Spine J. 2013 Jan 8;13(2):162–174. doi: 10.1016/j.spinee.2012.11.017

Figure 3. Expression profiling of cells released from Osteocel Plus.

Figure 3

(A) MSC-focused qPCR array results of Osteocel Plus cells versus huMSCs cultured for 7 days in vitro in MSC expansion media (top cluster panel). The Osteogenesis-focused qPCR array results of Osteocel Plus cells versus huMSCs cultured in osteogenic differentiation media for 28 days in vitro (bottom cluster panel). Red= increase in expression relative to huMSCs; Green= decrease in expression relative to huMSCs. (B) Relative expression of selected MSC genes from the MSC PCR array of Osteocel Plus cells compared to huMSCs. Both were cultured in MSC media 7 days prior to analysis. (C) Relative expression of osteogenesis-related genes BMP 2-7 (i-vi), alkaline phosphate (vii), Smad 1-4 (viii-xi), Runx2 (xii), Col1a1 (xiii), col4a3 (xiv), osteocalcin (xv) in MSC- and osteogenesis- focused qPCR arrays. Osteocel Plus cells were cultured in MSC and ODM media for 7 and 28 days in vitro, respectively (n=3, *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001; 95% Confidence interval of difference).