Figure 4. Proteomic profiling of Osteocel Plus cells cultured for 7 days in MSC media.
(A) Immunofluorescent staining (20x magnification) for early stem and progenitor cell markers and transcription factors: Stro-1 (Alexa 488), CD44 (Cy3), Runx2 (Alexa 488), BMP2 (Cy3), SMAD 1,4 (Cy3), and nuclear DAPI (Blue). (B) Flow cytometry analysis for stem cell markers CD44, Stro-1, CD90/thy1, CD146, and the mature osteoblast marker Osteocalcin.