Table 1. Inoculation methods, type of Leptosphaeria maculans inoculum, number of plants inoculated, design of experiments and assessment methods used in each of the controlled environment experiments with the doubled haploid (DH) lines A30 or C119.

Exp.	Inoculum1	Design	No. of plants inoculated2	Assessment method
L. maculans growth in leaf, leaf lamina inoculation
LExpt 1	Conidia (GFP)	Randomised block	8×2 plants, 3 leaves per plant	Lesion area3, distance grown (DG) along vein/petiole viewed by GFP
LExpt 2	Conidia (GFP)	Complete randomised	8 plants, 3 leaves per plant	Lesion area, DG viewed by GFP
LExpt 3	Ascospores	Randomised block	5×2 plants, 3 leaves per plant	DG assessed by extent of necrosis, L. maculans DNA in leaf petiole (qPCR)
LExpt 4	Ascospores	Complete randomised	12 plants, 3 leaves per plant	DG assessed by extent of necrosis, qPCR
LExpt 5	Ascospores	Complete randomised	8 plants, 2 leaves per plant	Lesion area, DG assessed by extent of necrosis, qPCR
L. maculans growth in stem, leaf petiole inoculation
PExpt 1	Conidia (GFP)	Complete randomised	10 plants, 2 petioles per plant	Stem canker score, extent of L. maculans growth in stem viewed by GFP
PExpt 2	Ascospores	Complete randomised	5 plants, 3 petioles per plant	Stem canker score, L. maculans DNA in stem (qPCR)
PExpt 3	Ascospores	Complete randomised	12 plants, 2 petioles per plant	Stem canker score, qPCR
PExpt 4	Ascospores	Complete randomised	12 plants, 2 petioles per plant	Stem canker score, qPCR

¹ L. maculans ascospores obtained from naturally infected oilseed rape stem base debris collected in August 2007; conidia were produced by GFP-transformed isolate ME24/3.13.

²Plants were inoculated when they had three fully expanded leaves. For details of leaf lamina or leaf petiole inoculation, see Fig. 2. All experiments were done at 20°C with alternating 12 h light/12 h darkness.

³The lesion area was estimated by multiplying the lesion length by lesion width.