Skip to main content
. 2014 Jan 15;9(1):e84895. doi: 10.1371/journal.pone.0084895

Figure 6. shCD5EμTg and non-transgenic mice response to immunization with T-dependent and T-independent (type 1 and type 2) antigens.

Figure 6

Mice were immunized i.p. with 50 µg TNP5-KLH (A), TNP0.3-LPS (B) or TNP65-Ficoll (C) prepared in 400 µl of PBS in complete Freund's adjuvant at day 0 and in 400 µl vehicle at day 14, as examples of TD, TI type 1 and TI type 2 antigens, respectively. Sera from immunized mice were collected at days 0, 7 and 14 (for TI response) or 0, 7, 14 and 21 (for TD response) after the primary immunization and levels of TNP-specific antibodies determined by ELISA. Ig levels are expressed as OD 450 nm values. The bars represent the average value for each group. ** p<0.05 *** p<0.01.