Figure 8. Delayed tumour growth in shCD5EμTg mice.
A) shCD5EμTg mice (n = 16) and non-transgenic littermates (n = 15) were injected s.c. with 5×104 B16 melanoma cells. Tumour growth was recorded over time by measuring with a Vernier calliper. *** p = 0.0177. B) Experimental metastasis assay. Mice were injected i.v. with 1×105 B16 melanoma cells. Fifteen days later, mice were sacrificed, their lungs removed and the metastases on the surface counted. p = 0.0994. C) Non-transgenic C57Bl/6 mice (n = 5 for each group) were injected with B16 cells as before. Chemotherapy (vincristine 0.5 mg/kg, Doxorubicin 3.3 mg/kg) was administered i.p. at day 3 after tumor cell injection. Additionally, rshCD5 (25 µg) or vehicle was injected i.p. every 48 hs for the duration of the experiment (20 days). ** p = 0.0083.