Skip to main content
. 2014 Jan 15;9(1):e84881. doi: 10.1371/journal.pone.0084881

Figure 1. High-throughput Screening and Characterization of the Two Hit Compounds.

Figure 1

(A) Schematic diagram of the screening strategy to induce the differentiation of human iPSCs (hiPSCs) into intermediate mesoderm (IM). Stage 1 indicates the differentiation step from hiPSCs into mesendoderm. Stage 2 indicates differentiation from mesendoderm into IM. Chemical screening was performed on Stage 2 cells. (B) The chemical structures of the two hit compounds, AM580 and TTNPB. (C) Results of flow cytometric analyses of the induction of OSR1+ cells on culture day 8; treatments consisted of two days of Stage 1 with CHIR99021 and activin A, and five days of Stage 2 with AM580 or TTNPB, DMSO (negative control), or combined CHIR99021 and BMP7 (positive control). (D) Dose-response curve of OSR1+ cell induction on day 8 by AM580 and TTNPB. The data in (C) and (D) are means±SD of three independent experiments (n = 3).