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. 2014 Jan 15;9(1):e84881. doi: 10.1371/journal.pone.0084881

Figure 4. The Small Molecule Method Functions via RARβ Receptors.

Figure 4

(A) Results of the flow cytometric analyses showing induction of OSR1+ cells by the small molecule method, when AM580 or TTNPB was replaced by all-trans retinoic acid (ATRA), adapalene, and CD1530. (B) Effects of adding RAR antagonists, BMS493, LE135, and MM11253, on the induction of OSR1+ cells by the TTNPB method. (C) The knockdown efficiency of siRNAs targeting RARB, and (D and E) effects on expression levels of OSR1 and the induction of OSR1+ cells by the TTNPB method. (F) Effects of adding a pan-RXR agonist (SR11237) or a pan-RXR antagonist (UVI3003) to the induction efficiency of OSR1+ cells by the TTNPB method. (G) The induction of OSR1+ cells by the small molecule method, when SR11237 replaced AM580 or TTNPB. (H) Results of qRT-PCR analyses showing OSR1 expression activated by the AM580 and TTNPB methods, and when ATRA, adapalene, or CD1530 were used instead of AM580 or TTNPB. OSR1-GFP knock-in hiPSCs prior to treatments were used to normalize the data. The data in (A–H) are presented as the mean±SD on culture day 6 of three independent experiments (n = 3).