Table 1. Eighty-four fungal isolates from the coarse outer hair of nine individuals sloths (B. varieagatus), their top BLAST matches, and maximum identity value from BLAST searches; group ID and tree (Fig. 1) revealing phylogenetic placement; and taxonomic placement based on phylogenetic analyses at the family (order) and genus levels (Fig. 1).
| Fungus ID | Sloth hair Sample # | Top BLAST Hit, (Accession Number) | Max ID (%) | Group ID | Tree | Family (Order) | Genus | OTU |
| F4847 | S006 | Fungal sp., (HQ832955.1)¥ | 99 | 18 | B | Apiosporaceae (Incertae sedis) | Arthrinium | S* |
| F4821 | S007 | Bionectria cf. ochroleuca, (EU552110.1)¥ | 99 | 3 | P | Bionectriaceae (Hypocreales) | Bionectria | I |
| F4889 | S008 | Bionectria cf. ochroleuca, (EU552110.1)¥ | 99 | 3 | P | Bionectriaceae (Hypocreales) | Bionectria | I |
| F4913 | S009 | Bionectria cf. ochroleuca, (EU552110.1)¥ | 99 | 3 | P | Bionectriaceae (Hypocreales) | Bionectria | I |
| F4874 | S005 | Bionectria pityrodes, (JQ411387.1)¥ | 99 | 3 | P | Bionectriaceae (Hypocreales) | Bionectria | W* |
| F4806 | S008 | Colletotrichum sp., (DQ300348.1)¥ | 99 | 7 | F | Glomerellaceae (Glomerellales) | Colletotrichum | R* |
| F4839 | S002 | Ascomycota sp., (HQ608154.1) [58] | 99 | 10 | K | Valsaceae (Diaporthales) | Cytospora | D |
| F4818 | S002 | Sordariomycetes sp., (JX174122.1)¥ | 99 | 10 | K | Valsaceae (Diaporthales) | Cytospora | D |
| F4801 | S003 | Endomelanconiopsis endophytica, (GQ469968.1)¥ | 100 | 5 | E | Botryosphaeriaceae (Botryosphaeriales) | Endomelanconiopsis | U* |
| F5071 | S003 | Fungal sp., (FJ612981.1) [23] | 99 | 15 | H | Xylariaceae (Xylariales) | Entonaema | N* |
| F4876 | S006 | Fungal sp., (EU563515.1) [59] | 99 | 13 | N | Nectriaceae (Hypocreales) | Fusarium | X |
| F4828 | S005 | Fungal sp., (FJ612934.1) [23] | 99 | 12 | M | Nectriaceae (Hypocreales) | Fusarium | O* |
| F4898 | S003 | Fungal sp., (FJ613085.1) [23] | 100 | 12 | M | Nectriaceae (Hypocreales) | Fusarium | Y* |
| F4881 | S009 | Fusarium concolor, (GQ505763.1) [60] | 99 | 13 | N | Nectriaceae (Hypocreales) | Fusarium | X |
| F4897 | S003 | Uncultured soil fungus clone, (DQ420777.1) [35] | 98 | 11 | L | Nectriaceae (Hypocreales) | Fusarium | AA* |
| F4877 | S003 | Uncultured soil fungus clone, (DQ420802.1) [35] | 99 | 11 | L | Nectriaceae (Hypocreales) | Fusarium | K |
| F4890 | S008 | Uncultured soil fungus clone, (DQ420802.1) [35] | 98 | 11 | L | Nectriaceae (Hypocreales) | Fusarium | K |
| F4900 | S004 | Fungal sp., (FJ613088.1) [23] | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | E |
| F4812 | S001 | Hypocrea jecorina, (AF510497.1)¥ | 99 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4813 | S001 | Hypocrea jecorina, (EU280094.1) [61] | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4815 | S001 | Hypocrea jecorina, (EU280094.1) [61] | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4814 | S001 | Hypocrea jecorina, (JQ070073.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4852 | S001 | Hypocrea jecorina, (JQ070073.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4853 | S001 | Hypocrea jecorina, (JQ070073.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4855 | S001 | Hypocrea jecorina, (JQ070073.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4856 | S001 | Hypocrea jecorina, (JQ070073.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4854 | S001 | Hypocrea jecorina, (JQ411369.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4882 | S009 | Hypocrea jecorina, (JQ411369.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4901 | S004 | Hypocrea lixii, (AY605743.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | E |
| F4831 | S005 | Hypocrea lixii, (JF923806.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | E |
| F4902 | S005 | Hypocrea lixii, (JF923806.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | E |
| F4873 | S005 | Hypocrea muroiana, (JN943367.1) [62] | 99 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | H |
| F4883 | S006 | Hypocrea nigricans, (JN943369.1) [62] | 99 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | E |
| F4816 | S001 | Trichoderma reesei, (JQ979435.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | A |
| F4830 | S005 | Uncultured Trichoderma clone, (JX317342.1)¥ | 100 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | H |
| F4845 | S005 | Uncultured Trichoderma clone, (JX317342.1)¥ | 99 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | H |
| F4860 | S005 | Uncultured Trichoderma clone, (JX317342.1)¥ | 99 | 1 | I | Hypocreaceae (Hypocreales) | Hypocrea | H |
| F4825 | S004 | Lasiodiplodia parva, (GQ469961.1)¥ | 100 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F4844 | S004 | Lasiodiplodia parva, (GQ469961.1)¥ | 100 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F4802 | S004 | Lasiodiplodia parva, (GQ469964.1)¥ | 100 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F5068 | S009 | Lasiodiplodia theobromae, (FJ478103.1)¥ | 99 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F4807 | S008 | Lasiodiplodia theobromae, (GQ469934.1)¥ | 100 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F4906 | S007 | Lasiodiplodia theobromae, (GQ469934.1)¥ | 100 | 4 | Q | Botryosphaeriaceae (Botryosphaeriales) | Lasiodiplodia | C |
| F4904 | S006 | Fungal sp., (GU566256.1) [63] | 99 | 14 | S | Leptosphaeriaceae (Pleosporales) | Leptosphaeria | J |
| F4905 | S006 | Fungal sp., (GU566256.1) [63] | 100 | 14 | S | Leptosphaeriaceae (Pleosporales) | Leptosphaeria | J |
| F4819 | S006 | Fungal endophyte sp., (EU977287.1) [55] | 99 | 20 | D | Lophiostomataceae (Pleosporales) | Unknown | P* |
| F4831a | S002 | Fungal endophyte sp., (EU561602.1)¥ | 99 | 19 | C | Montagnulaceae (Pleosporales) | Paraconiothyrium † | A |
| F4884 | S008 | Penicillium herquei, (EU833220.1)¥ | 99 | 6 | O | Trichocomaceae (Eurotiales) | Penicillium | V* |
| F4863 | S009 | Penicillium sp., (JF288548.1) [64] | 99 | 6 | O | Trichocomaceae (Eurotiales) | Penicillium | T* |
| F4803 | S004 | Fungal endophyte sp., (EU561622.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4846 | S006 | Fungal endophyte sp., (EU561622.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4878 | S008 | Fungal endophyte sp., (EU561622.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4896 | S002 | Fungal endophyte sp., (EU561622.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4842 | S003 | Pestalotiopsis microspora, (FJ478120.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4848 | S006 | Pestalotiopsis microspora, (FJ478120.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4872 | S004 | Pestalotiopsis sp., (EU605882.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4820 | S006 | Pestalotiopsis sp., [EU605882.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4824 | S003 | Pestalotiopsis sp., (EU605882.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4829 | S005 | Pestalotiopsis sp., (EU605882.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4858 | S004 | Pestalotiopsis sp., (EU605882.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4908 | S007 | Pestalotiopsis sp., (EU605882.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4857 | S002 | Pestalotiopsis sp., (EU605882.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | Q* |
| F4817 | S002 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4826 | S004 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4827 | S005 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4837 | S002 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4841 | S003 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4861 | S005 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4862 | S007 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4895 | S002 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4909 | S008 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F5074 | S003 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4823 | S003 | Pestalotiopsis sp., (HQ832816.1)¥ | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4894 | S002 | Pestalotiopsis theae, (HQ832793.1)¥ | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4870 | S002 | Uncultured fungus clone, (JN890176.1) [65] | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | G |
| F4875 | S005 | Uncultured fungus clone, (JN890258.1) [65] | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F4879 | S008 | Uncultured fungus clone, (JN890258.1) [65] | 99 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F5069 | S006 | Uncultured fungus clone, (JN890258.1) [65] | 100 | 2 | J | Amphisphaeriaceae (Xylariales) | Pestalotiopsis | B |
| F5073 | S008 | Sordariomycetes sp., (JQ760525.1) [65] | 99 | 17 | A | Calosphaeriaceae (Calosphaeriales) | Phaeoacremonium | L* |
| F5070 | S006 | Uncultured fungus clone, (GU721790.1)¥ | 99 | 16 | T | Cephalotheceae (Sordariales) | Phialemonium | F |
| F5072 | S003 | Uncultured fungus clone, (GU721790.1)¥ | 99 | 16 | T | Cephalotheceae (Sordariales) | Phialemonium | F |
| F4886 | S004 | Robillarda sessilis, (HQ608017.1) [58] | 99 | 8 | G | Amphisphaeriaceae (Xylariales) | Robillarda † | Z* |
| F4891 | S009 | Phoma sp., (JQ621876.1)¥ | 99 | 9 | R | Pleosporales† | Unknown | BB* |
| F4850 | S009 | Uncultured soil fungus clone, (DQ421180.1) [35] | 96 | 9 | R | Unknown | Unknown | M* |
OTU codes indicate operational taxonomic units based on 95% sequence similarity. Order and family level identifications were confidently assigned to 82 isolates and genus level identifications were confidently assigned to 80 isolates.
Unpublished strains.
*OTUs occurring only once (singletons).
Tentative phylogenetic placement.