Table 4. Minimum inhibitory concentrations (MIC) of 5 sloth hair associated fungal extracts in the BioMAP antibiotic profile screen [30].
Minimum Inhibitory Concentration (Normalized 0–1) | |||||
Fungus ID | F4806 | F4807 | F4818 | F4844 | F4898 |
Bacillus subtilis | 1 | 0.85 | 1 | 1 | 1 |
Staphylococcus epidermis | 0.46 | 0 | 0.88 | 0.87 | 0.69 |
Enterococcus faecium | 0.6 | 0 | 0.52 | 0.6 | 0.53 |
Listeria ivanovii | 0.6 | 0 | 0.4 | 0.46 | 0.53 |
Staphylococcus aureus | 0.87 | 0 | 1 | 0.6 | 0.85 |
MRSA | - | - | - | - | - |
Yersinia pseudotuberculosis | 0 | 0.69 | 0 | 0 | 0 |
Pseudomonas aeruginosa | 0 | 0 | 0 | 0 | 0 |
Salmonella typhimerium | 0 | 0.53 | 0 | 0 | 0 |
Vibrio cholerae | 0 | 0 | 0 | 0 | 0 |
Escherichia coli | 0 | 0.69 | 0 | 0 | 0 |
Acinetobacter baumanii | 0 | 1 | 0 | 0 | 0 |
Enterobacter aerogenes | 0 | 0 | 0 | 0 | 0 |
Ochrobactrum anthropi | 0 | 0.69 | 0 | 0 | 0 |
Providencia alcalifaciens | 0 | 0.53 | 0 | 0 | 0 |
To generate a concentration- independent bioactivity profile, raw assay results were normalized giving a range of values from 0 (inactive) to 1 (most bioactive). These bioactivity fingerprints were then compared to fingerprints of known antibiotics from all the major structural classes, which had previously been tested in the BioMAP assay. The bioactivity fingerprint of F4807 was of particular interest as it did not match that of any antibiotic previously tested in the BioMAP screen.
- = Test failed, data not available.