Table 4. Comparisons of predicted essential enzymes encoding genes shared by all three Category A agents to experimentally identified essential genes across various pathogenic bacteria.
| Sl | Enzyme | Gene | Vc | Bs | Ec | Hi | Sp | Fn | Ab | Pa | Se | Sa | Mg | Mt | Mp | Hp |
| 1 | 4.1.1.36/6.3.2.5 | Dfp (coaB, coaC) | − | − | + | − | + | + | + | − | + | − | − | + | − | − |
| 2 | 2.7.7.3 | coaD | + | − | + | + | + | + | + | + | + | + | − | − | − | − |
| 3 | 2.7.1.24 | coaE | + | − | + | + | − | − | − | + | + | + | − | + | − | − |
| 4 | 4.1.2.25 | folB | − | − | − | − | − | + | + | + | + | − | − | + | − | + |
| 5 | 6.3.2.12/6.3.2.17 | folC | − | − | + | − | − | − | + | − | + | − | − | + | − | − |
| 6 | 3.5.4.16 | folE | + | − | + | − | − | − | + | − | + | − | − | + | − | + |
| 7 | 2.7.4.8 | Gmk | − | + | + | + | − | + | + | − | − | + | − | − | + | − |
| 8 | 2.7.4.9 | Tmk | + | + | + | + | − | − | − | + | + | + | + | − | + | − |
| 9 | 4.1.1.65 | Psd | − | − | + | − | − | + | + | + | + | − | − | − | − | − |
Vc: Vibrio cholera N116961, Bs: Bacillus subtilis 168, Ec: E. coli MG1655, Hi: Haemophilus influenzae Rd KW20, Sp: Streptococcus pneumoniae, Fn: Francisella tularensis sub spp novicida U112, Ab: Acinetobacter baylyi ADP1, Pa: Pseudomonas aeruginosa UCBPP-PA14, Se: Salmonella enterica serv Typhi, Sa: Staphylococcus aureus N315, Mg: Mycoplasma genitalium G37, Mt: Mycobacterium tuberculosis H37Rv, Mp: Mycoplasma pulmonis UAB CTIP and Hp: Helicobacter pylori 26695. “+” Indicates as essential, “− ” Indicates as non-essential.