Figure 2. Monocyte characterization in mixed chimeric wt/IL-17 receptor A deficient mice.

Monocyte analysis in mixed bone marrow chimeric wt(CD45.1)/Il17ra^-/-(CD45.2) mice (recipients: CD45.2 wt mice). (A) Monocytes were gated by CD115 and IL-17 receptor expression was assessed as control of complete chimerism. (B) Gr1^high and Gr1^low monocytes in mixed chimeric mice were analyzed for the intensity of Il17ra expression (MFI, n = 16 from 3 independent transplantations, Bonferroni after One-way-ANOVA). (C) Gr1^high monocytes were sorted from bone-marrow and expression of IL-17 receptor subunits analyzed by qPCR (means of n = 2 independent cell sorts).