Figure 7. Kinetics of labeled Gr1^high and Gr1^low monocytes in the presence and absence of Il17ra^-/-.

(A,B) Monocytes in mixed chimeric wt/Il17ra^-/- mice were labeled with fluorescent latex beads (A). The proportion of labeled CD11b⁺CD115⁺ cells among each monocyte subgroup (Gr1^high and Gr1^low monocytes) within each genotype was assessed over time (B, n = 4-5, 2 independent transplantations, Bonferroni after ANOVA). (C) Dividing cells in mixed bone marrow chimeric wt/Il17ra^-/- mice labeled with a single BrdU injection and peripheral blood monocytes were analyzed for BrdU incorporation at the indicated timepoints. In every animal, the proportion of labeled cells among Gr1^high and Gr1^low monocytes of either genotype was assessed over time (n = 4, * indicates sign. differences between wt and Il17ra^-/- Gr1^low monocytes at the indicated timepoints, # indicates a sign. change over time within wt (black bars) or Il17ra^-/- (white bars) Gr1^low monocytes, analysis with Bonferroni after ANOVA). (D) Proliferation of bone marrow Gr1^high and Gr1^low monocytes of both genotypes was assessed 20 h after BrdU injection (n = 7 from 2 independent transplantations, Bonferroni after ANOVA).