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. 2014 Jan 15;9(1):e85454. doi: 10.1371/journal.pone.0085454

Figure 4. Imprinting analysis of IGF2, PHLDA2 and IGF2R.

Figure 4

Representative sequencing chromatograms are shown from one of the 40 informative CVS samples for the IGF2 A/G polymorphism (rs680) (A, B), one (from 21) for the PHLDA2 A/G polymorphism (rs1056819) (C, D) and two (from 24) for the IGF2R A/G polymorphism (rs1805075) (E, F, G, H). Heterozygous genomic CVS DNA sequence is shown in A), C), E) and G). While monoallelic expression from the corresponding cDNA samples are shown in B), D) and H). Monoallelic expression in cDNA was found in all informative IGF2 and PHLDA2 samples as well as 12% of IGF2R samples. Biallelic expression, as shown in F), was found in the majority (88%) of informative cDNA samples for IGF2R.