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. 2014 Jan 15;9(1):e85389. doi: 10.1371/journal.pone.0085389

Figure 1. miR-24 inhibits the intrinsic apoptosis pathway.

Figure 1

(A) FACS analysis on AnnexinV+PI– - apoptotic cells treated with Camp with (middle panel) or without miR-24 mimic (right panel). The control group is shown in the left panel. Camp., Camptothecin (B) Quantification on AnnexinV+PI– cells treated with Fas or Camp with or without miR-24 mimic. (C) Inhibition of Caspase 9, Caspase 12 or Caspase 3, but not caspase 8, rescued the miR-24 loss-of-function phenotype. Cells were incubated with Z-IETD-FMK, Z-LEHD-FMK, Z-ATAD-FMK or Z-VAD-FMK for assessment of caspase-8, -9, -12 and pan caspases activity, respectively. (D) Knockdown of Caspase 9, Caspase 12 or Caspase 3, but not Caspase 8, rescued the increased apoptosis caused by inhibition of miR-24. *, p<0.05; **, p<0.01. Data were analyzed by unpaired Student's t test. (E) Validation of knockdown efficiency using siRNA cocktail against Caspase 3, 8, 9 and 12 by qPCR. RQ, Relative Quantification. Error bars represent SEM.