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. 2014 Jan 15;9(1):e85389. doi: 10.1371/journal.pone.0085389

Figure 4. miR-24 regulates mitochondria-mediated apoptosis pathway.

Figure 4

(A) Overexpression of miR-24 suppresses cytochrome C release from mitochondria to cytosol. Western blot for cyto.C in cytosol (C) or mitochondria (M) fractions of primary cardiomyocytes transfected with miR-24 mimic, inhibitor, or corresponding controls. Caspase 8 was used as a loading control for cytosol fraction; CoxIV was used as a protein marker for the mitochondrial fraction. (B) qPCR for Bax mRNA from primary cardiomyocytes transfected with miR-24 mimic, inhibitor and corresponding controls. (C) Western blot for Bax on cytosol (c) or mitochondrial (m) fractions of primary cardiomyocytes transfected with miR-24 mimic, inhibitor and corresponding controls. (D) Western blot for Bax on cytosol (c) or mitochondrial (m) fractions of primary cardiomyocytes transfected with control or Bim siRNAs. (E) qPCR for Bcl2 mRNA from cells transfected with miR-24 mimic, inhibitor or corresponding controls. (F) Western blot for Bcl2 protein showing miR-24 did not regulate the protein level of Bcl2.