Skip to main content
. 2014 Jan 15;9(1):e85358. doi: 10.1371/journal.pone.0085358

Figure 2. Amino acid substitution in Domain IV of Osiaa23 prevents the protein-protein interactions between Osiaa23 and OsARFs.

Figure 2

Interactions between Osiaa23 and OsARFs, Osiaa23-R5 and OsARFs in the yeast two-hybrid system. 1, positive control; 2, negative control; 3, Osiaa23 + OsARF6; 4, Osiaa23 + OsARF12; 5, Osiss23 + OsARF16; 6, Osiaa23 + OsARF17; 7, Osiaa23 + OsARF25; 8, Osiaa23-R5 + OsARF6; 9, Osiaa23-R5 + OsARF12; 10, Osiaa23-R5 + OsARF16; 11, Osiaa23-R5 + OsARF17; 12, Osiaa23-R5 + OsARF25. Yeast was grown on medium without leucine and tryptophan (SD -Leu/ -Trp) as a contral (A) and medium without leucine, tryptophan, histidine and ade (SD -Trp/ -Leu/ -Ade/ -His) to test the protein-protein interactions (B).