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Figure 3. Kd values as a function of on-plate incubation time, obtained from fitting Equation 1 to the data in Figure 2 for the low concentration Fab/ligand samples (squares) and the high concentration Fab/ligand samples (circles). Higher Kd values suggest potential exchange from ligand bound to Fab in solution to the anti-ligand antibody on the plate, leading to an overestimation of the free ligand.
