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. 2014 Jan 15;9(1):e85644. doi: 10.1371/journal.pone.0085644

Figure 7. Results of the aggregation assay using collagen fibrils from the dermis of H. leucospilota.

Figure 7

(A,B) The effect of the softener and (C,D) the effect of the repetitive freeze-thaw treatment on aggregation induced by tensilin. Samples were observed under a light microscope. Scale bars, 1 mm. (A) H-tensilin caused a large compact aggregate when applied to the suspending solution of collagen fibrils. (B) The introduction of the softener to the solution with a tensilin-induced large aggregate. The aggregate dissolved into small fluffy aggregates. (C) Control to D. The tensilin-induced aggregate was maintained in the solution with tensilin when it was stored at 4°C. (D) After the repetitive freeze-thaw treatment, the large compact aggregate dissolved into small fluffy aggregates.