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. 2014 Jan 15;9(1):e86113. doi: 10.1371/journal.pone.0086113

Figure 5. Immunodetection of the 19-kDa urease beta subunit in total protein extracts of Y. enterocolitica.

Figure 5

The relative signal for each band compared to wild type (which was set to 100%) is indicated. Upper panel shows the immunoblot, bottom panel shows part of the Coomassie blue-stained gel used as loading control. (A) Loading was as follows: 1, WA-314; 2, SOR4; 3, SOR3; 4, urease-negative control strain 8081-U-GB; 5, JB580v, and 6, SOR17. (B) Complementation analysis. Loading was as follows: 1, SOR4(pACYC184ts); 2, SOR4(pAhfq); 3, WA-314(pAhfq); 4, WA-314(pACYC184ts); 5, WA-314; and 6, 8081-U-GB. In another experiment, we also observed restoration of the production of UreB in the hfq-negative strain SOR17 carrying pAhfq (data not shown).