Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Bone Miner Res. 2014 Feb;29(2):304–315. doi: 10.1002/jbmr.2038

Figure 3. Effects of muscular dystrophy on osteoclasts during fracture repair.

Figure 3

(A) Stereological quantification of TRAP+ osteoclasts within wild type (WT) and mdx calluses after 5 days (d5) and 7 days (d7) of bone regeneration. (B) Safranin-O (SO) staining and representative TRAP staining (arrows, area corresponds to dashed box in SO staining) on adjacent sections of WT and mdx fracture calluses at day 5 (d5) and day 7 (d7) of bone regeneration. (C) Relative quantification (RQ) by RTqPCR of matrix metalloproteinase-9 (mmp9) (right) mRNA, marker of osteoclasts, within WT and mdx calluses at day 7 (d7) and day 14 (d14). Expression level was normalized to GAPDH mRNA. (D) Representative TRAP staining in WT and mdx fracture calluses at day 14 (d14) and day 21 (d21) of bone regeneration. Error bars represent ±SEM. Student’s t test, P values *p<0.05, **p<0.001 (n=5 or 6 per group for TRAP staining, n=3 per group for gene expression). Scale bar: 1mm, 100 µ (B).