Figure 5.

The increased synaptic and I-IC activity observed in slices derived from young presymptomatic Syn II(−) mice depends on synaptic inhibition. A, Application 50 μm Ptx eliminates inhibitory transmission. Recording were made at −65 mV holding potential in cesium-based solution in the presence of CNQX and AP-5. B, Spontaneous glutamatergic activity is comparable in WT and SynII(−) slices when inhibitory transmission is blocked by Ptx. C, Ptx application occludes the difference in sEPSC frequency between the WT and SynII(−) slices. D, Ptx application occludes the difference in I-IC activity between the WT and SynII(−) slices. *Significant difference (p < 0.05 per ANOVA followed by post hoc Tukey) between genotypes in the absence of Ptx. Data collected from 6 cells (3 animals) per genotype. Spontaneous activity was recorded for 5 min in the absence and for 5 min in the presence of Ptx.