Figure 9.

Lack of effect of 4-AP or elevated extracellular Ca²⁺ on EPSC/IPSC amplitudes at SynII(−)/Rab3a(−) synapses. A, In SynII(−)/Rab3a(−), DKO synapses in slices derived from young mice 4-AP (10 μm) fails to produce a detectable increase in either EPSC (A₁, A₃, p = 0.43, n = 6) or IPSC amplitude (A₂, A₃, p = 0.50, n = 5). B, In Syn II(−)/Rab3a(−), DKO synapses elevating extracellular calcium to 5 mm fails to elicit a statistically significant increase in either EPSCs (B₁, B₃, p = 0.48, n = 10) or IPSCs (B₂, B₃, p = 0.48, n = 8). C, Bar graph summarizing the results of 4-AP (C₁) and elevated calcium (C₂) on EPSCs and IPSCs in the different genotypes. These plots summarize the data presented in Figures 3, 4, 8, and 9A, B.