Figure 1.

Histological appearance of apoptotic profiles in frontal subcortical white matter (WM) of alcohol-exposed G120 fetal NHP brain. Panel (a) depicts the appearance of degenerating WM cells stained with antibodies to AC3, and panels (b) and (c) are from adjacent sections stained with silver or antibodies to fractin. All three of these cell death markers detect a similar number of degenerating profiles in the same WM location, and the dying cells have a similar morphological appearance, regardless whether stained by AC3, silver or fractin. Panel (d) displays a WM scene from the same brain immunofluorescently double stained with antibodies to AC3 and GFAP, showing three astrocytes stained with GFAP (green) beside a dying cell stained by AC3 (red). The dying cell is marked by AC3 but not GFAP, signifying that it is not an astrocyte. Scale bar in d = 15 μM for all four panels.