Table 3.

PCR primers used for the detection of STEC virulence or adherence genes

Targets	Primer	Oligonucleotide sequence (5′-3′)	Amplicon size (bp)	Reference
stx1	stx 1 -F	AAATCGCCATTCGTTGACTACTTCT	370	[61]
	stx 1 -R	TGCCATTCTGGCAACTCGCGATGCA
stx2	stx 2 -F	CAGTCGTCACTCACTGGTTTCATCA	283	[61]
	stx 2 -R	GGATATTCTCCCCACTCTGACACC
stx2e	stx 2e -F	CGGAGTATCGGGGAGAGGC	411	[62]
	stx 2e -R	CTTCCTGACACCTTCACAGTAAAGGT
SLT-II	GK1	ATGAAGTGTATATTATTTAAATGG	1241	[63]
	GK4	TCAGTCATTATTAAACTGCAC
ehxA	ehxA-F	GGTGCAGCAGAAAAAGTTGTAG	1551	[64]
	ehxA-R	TCTCGCCTGATAGTGTTTGGTA
hlyA	hlyA1-F	GACAAAGCACGAAAGATG	2930	[6]
	hlyA2-R	CAACTGCAATAAAGAAGC
astA	EAST11a	CCATCAACACAGTATATCCGA	111	[65]
	EAST11b	GGTCGCGAGTGACGGCTTTGT
irp2	irp2-F	AAGGATTCGCTGTTACCGGAC	280	[66]
	irp2-R	TCGTCGGGCAGCGTTTCTTCT
fyuA	fyuA-F	TGATTAACCCCGCGACGGGAA	880	[66]
	fyuA-R	CGCAGTAGGCACGATGTTGTA
eae	eae-F	ACGTTGCAGCATGGGTAACTC	815	[36]
	eae-R	GATCGGCAACAGTTTCACCTG
paa	M155-F1	ATGAGGAAACATAATGGCAGG	350	[67]
	M155-R1	TCTGGTCAGGTCGTCAATAC
iha	iha-F	CAGTTCAGTTTCGCATTCACC	1305	[68]
	iha-R	GTATGGCTCTGATGCGATG
saa	saa-F	CGTGATGAACAGGCTATTGC	119	[14]
	saa-R	ATGGACATGCCTGTGGCAAC
toxB	toxB-F	ATACCTACCTGCTCTGGATTGA	602	[69]
	toxB-R	TTCTTACCTGATCTGATGCAGC
efa1	efa1-F	GAGACTGCCAGAGAAAG	479	[11]
	efa1-R	GGTATTGTTGCATGTTCAG
lpfAO157/OI-154	lpfAO157/OI-154-F	GCAGGTCACCTACAGGCGGC	525	[14]
	lpfAO157/OI-154-R	CTGCGAGTCGGCGTTAGCTG
lpfAO157/OI-141	lpfAO157/OI-141-F	CTGCGCATTGCCGTAAC	412	[70]
	lpfAO157/OI-141-R	ATTTACAGGCGAGATCGTG
lpfAO113	lpfAO113-F	ATGAAGCGTAATATTATAG	573	[9]
	lpfAO113-R	TTATTTCTTATATTCGAC
F4(K88)	F4-F	GCTGCATCTGCTGCATCTGGTATGG	792	[15]
	F4-R	CCACTGAGTGCTGGTAGTTACAGCC
F5(K99)	F5-F	TGCGACTACCAATGCTTCTG	450	[15]
	F5-R	TATCCACCATTAGACGGAGC
F6(P987)	F6-F	TCTGCTCTTAAAGCTACTGG	333	[15]
	F6-R	AACTCCACCGTTTGTATCAG
F17	F17-F	GGGCTGACAGAGGAGGTGGGGC	411	[15]
	F17-R	CCCGGCGACAACTTCATCACCGG
F18	F18-F	GTGAAAAGACTAGTGTTTATTTC	510	[15]
	F18-R	CTTGTAAGTAACCGCGTAAGC
F41	F41-F	GAGGGACTTTCATCTTTTAG	431	[15]
	F41-R	AGTCCATTCCATTTATAGGC