Fig. 8.

AFLP marker analysis (EACT-MCAT) of genotypes TMS 60444 and SM 1219–9 and 29 anther-derived callus cell lines from donor plant SM 1219–9 of cassava (M. esculenta Crantz). Lanes 1, 25, and 34 correspond to the molecular weight markers used. Lanes 2–22 correspond to the cell lines derived from cold-pretreated (10 °C) anthers, while lanes 26–36 correspond to those from heat-pretreated (38 °C) ones. Lane 23 correspond to donor plant SM 1219–9, and lane 24, to the model transformation cassava genotype TMS 60444