Table 2.
Simple sequence repeat alleles detected using six SSRY primer pairs for eight callus cell lines derived from the donor plant SM 1219-9
| Marker | SSRY 19 | SSRY 21 | SSRY 63 | SSRY 82 | SSRY 164 | SSRY 182 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele sample | 1 | 2 | 3a | 1 | 2 | 1 | 2 | 1 | 2 | 3a | 1 | 2 | 3a | 1 | 2 |
| Donor | 230 | 234 | 180 | 188 | 300 | 312 | 209 | 226 | 183 | 185 | 254 | 256 | |||
| C 1 | + | − | − | + | + | − | + | − | + | − | + | − | |||
| C 2 | + | − | 206 | − | + | + | − | + | − | 211 | + | − | + | − | |
| C 3 | + | − | − | + | + | − | + | − | 211 | + | − | + | − | ||
| C 4 | + | − | − | + | + | − | + | − | + | − | + | − | |||
| C 5 | x | x | − | + | x | x | x | x | x | x | x | x | |||
| C 6 | + | − | − | + | + | − | + | − | + | − | 157 | + | − | ||
| C7 | + | − | + | + | + | − | + | − | 211 | + | − | + | − | ||
| C 8 | x | x | x | x | x | x | + | − | 211 | x | x | x | x | ||
Size and the distribution of the homozygous vs. heterozygous alleles of the selected SSRY markers are shown for the donor plant SM 1219–9 and anther-derived callus cell lines; + indicates presence of allele presence, while −, absence of allele
aAn extra allele appeared in certain reactions of the analyzed calli indicating a chromosome deletion or rearrangement