Table 6.

Immunofluorescence analysis of arteries and veins in scrapie-infected and uninfected Tg44+/+ mice for detection of FITC-OVA at 30 min after microinjection of tracer

	Uninfected		Scrapie-infected
Vessels	N	%	N	%
Arteries	78	82	152	79
Veins	17	18	40	21
Total	95	100	192	100
A/V ratio	4.6 to 1		3.8 to 1

Data are from 3 mice per group; mice were analyzed between 250–280 days post-infection with RML scrapie as described in the Methods. Area counted: Uninfected =10.88 mm², scrapie-infected = 19.52 mm². Results similar to those in Tables 5 and 6 were also observed in uninfected C57BL/10 mice (data not shown). Data for arteries and veins for uninfected versus scrapie-infected Tg44+/+ mice were not significantly different by two-tailed Fischer’s exact test (p = 0.64). In contrast, there was a highly significant difference in numbers of arteries and veins associated with FITC-OVA (Table 6) versus PrPres (Table 2) in scrapie-infected mice (P < 0.0001) by two-tailed Fischer’s exact test.