Table 2.
Cases
| case ID | type | LI | SL | MLPA | MLPA 0-3 | MLPA >0.25 | DL | |
|---|---|---|---|---|---|---|---|---|
| 1 |
oligo |
7.567 |
1 |
0.6275 |
2 |
1 |
1 |
|
| 2 |
oligo |
13.700 |
1 |
0.675 |
2 |
1 |
1 |
|
| 3 |
oligo |
12.600 |
1 |
0.8075 |
3 |
1 |
1 |
|
| 4 |
oligo |
24.667 |
1 |
0.4725 |
1 |
1 |
1 |
|
| 5 |
oligo |
17.100 |
1 |
0.8525 |
3 |
1 |
1 |
|
| 6 |
oligo |
13.600 |
1 |
0.8025 |
3 |
1 |
1 |
|
| 7 |
oligo |
19.567 |
1 |
0.6425 |
2 |
1 |
1 |
|
| 8 |
oligo |
14.267 |
1 |
0.6925 |
2 |
1 |
1 |
|
| 9 |
oligo |
8.800 |
1 |
0.6525 |
2 |
1 |
1 |
|
| 10 |
oligo |
26.133 |
1 |
0.5925 |
2 |
1 |
*1 |
|
| 11 |
gbm |
56.367 |
0 |
0.1125 |
0 |
0 |
0 |
|
| 12 |
gbm |
19.167 |
1 |
0.8475 |
3 |
1 |
1 |
|
| 13 |
gbm |
84.400 |
0 |
0.09 |
0 |
0 |
0 |
|
| 14 |
gbm |
17.967 |
1 |
0.2625 |
1 |
1 |
*1 |
|
| 15 |
gbm |
57.700 |
0 |
0.255 |
1 |
1 |
0 |
|
| 16 |
gbm |
89.900 |
0 |
0.4075 |
1 |
1 |
0 |
|
| 17 |
gbm |
72.267 |
0 |
0.2075 |
0 |
0 |
0 |
|
| 18 |
gbm |
19.533 |
1 |
0.3525 |
1 |
1 |
*1 |
|
| 19 |
gbm |
54.133 |
0 |
0.42 |
1 |
1 |
*1 |
|
| 20 |
gbm |
53.900 |
0 |
0.1375 |
0 |
0 |
0 |
|
| 21 |
gbm |
21.967 |
1 |
0.485 |
1 |
1 |
1 |
|
| 22 |
gbm |
54.167 |
0 |
0.5475 |
2 |
1 |
1 |
|
| 23 |
gbm |
33.000 |
1 |
0.085 |
0 |
0 |
0 |
|
| 24 |
gbm |
76.300 |
0 |
0.2725 |
1 |
1 |
0 |
|
| 25 |
gbm |
96.733 |
0 |
0.24 |
0 |
0 |
0 |
|
| 26 |
gbm |
49.567 |
1 |
0.4275 |
1 |
1 |
1 |
|
| 27 |
gbm |
43.967 |
1 |
0.1375 |
0 |
0 |
*1 |
|
| 28 |
gbm |
40.533 |
1 |
0.1025 |
0 |
0 |
0 |
|
| 29 |
gbm |
7.233 |
1 |
0.1275 |
0 |
0 |
1 |
|
| 30 |
gbm |
39.067 |
1 |
0.5125 |
2 |
1 |
1 |
|
| 31 |
gbm |
25.833 |
1 |
0.2075 |
0 |
0 |
*1 |
|
| 32 |
gbm |
33.900 |
1 |
0.13 |
0 |
0 |
*1 |
|
| 33 |
gbm |
28.000 |
1 |
0.365 |
1 |
1 |
0 |
|
| 34 |
gbm |
45.000 |
1 |
0.275 |
1 |
1 |
1 |
|
| 35 |
gbm |
84.100 |
0 |
0.205 |
0 |
0 |
0 |
|
| 36 |
gbm |
85.100 |
0 |
0.2975 |
1 |
1 |
0 |
|
| 37 |
gbm |
64.933 |
0 |
0.11 |
0 |
0 |
0 |
|
| 38 |
gbm |
53.400 |
0 |
0.17 |
0 |
0 |
0 |
|
| 39 |
gbm |
6.800 |
1 |
0.825 |
3 |
1 |
1 |
|
| 40 | gbm | 22.500 | 1 | 0.2425 | 0 | 0 | 0 |
Case ID (identification) is the case number we assigned in this study. Tumour types were oligodendroglial tumours (oligo) and glioblastomas (gbm). The oligodendroglial tumours were oligodendroglioma WHO grade II (cases 4, 5 and 6), anaplastic oligodendroglioma WHO grade III (cases 1, 2, 3, 7 and 9), oligoastrocytoma WHO grade II (case 10) and anaplastic oligoastrocytoma WHO grade III (case 8). The glioblastomas were all glioblastoma WHO grade IV except cases 13 and 18 which were glioblastoma with oligodendroglioma component WHO grade IV. LI is the nuclear labelling index for single-labelled MGMT as determined by computerised image analysis. SL is the binarised score for the single-labelling (0 = LI > 50%, 1 = LI from 0 to 50%). MLPA is the MLPA ratio (please see text). MLPA 0–3 is the score for MLPA according to cut-offs for the MLPA ratio provided by Jeuken et al. 2007 [10] (0 = unmethylated, 1 = mildly methylated, 2 = moderately methylated and 3 = extensively methylated). MLPA > 0.25 is the binarised methylation score for MLPA (0 = unmethylated, 1 =methylated). DL is the binarised score for double labelling (0 = mainly MGMT-positive in tumour cells, 1 = mainly MGMT-negative in tumour cells). The asterisked cases were initially assigned a score of 1 in the tripartite system (please see text for explanation).