Table 1.
Primer sequences of target genes for qRT-PCR
| Target genes | Nucleotide sequences (5′-3′) | Product size | |
|---|---|---|---|
|
GAPDH |
Forward |
AGCCCCCAACACTGAGCAT |
111 bp |
| Reverse |
TGCAGCGAACTTTATTGATGGT |
||
|
Caspase-3 |
Forward |
TTCTCCCTGGACGCCACTT |
111 bp |
| Reverse |
CCTACCCCACTCCCAGTCATT |
||
|
iNOS |
Forward |
GTCTCTCCAAACCCCTCACTGT |
112 bp |
| Reverse |
GGAGCAAAAAAGGGCAACAC |
||
|
Bcl-2 |
Forward |
TGGGATGCCTTTGTGGAACT |
125 bp |
| Reverse |
CAGGTATGCACCCAGAGTGATG |
||
|
Bax |
Forward |
GAGCTGCAGAGGATGATTGCT |
128 bp |
| Reverse |
GCAAAGTAGAAGAGGGCAACCA |
||
|
Fas |
Forward |
GTGTGCAAGGCTCAAGGATGT |
120 bp |
| Reverse |
TGGGATGCCTTTGTGGAACT |
||
|
ATF-3 |
Forward |
TCTAGCCGCTCTCTGGACC |
147 bp |
| Reverse |
TCCTCAAACACCAGTGACCC |
||
|
JNK |
Forward |
TCCAGTTCTCGTACCCGCTA |
135 bp |
| Reverse |
AGCATGGCGTGACACAGTAA |
||
|
p38 |
Forward |
TTTGCTCAGTACCACGACCC |
107 bp |
| Reverse |
TCGTAGGTCAGGCTCTTCCA |
||
|
Akt |
Forward |
GCTGCGGCTCCTCATTCA |
125 bp |
| Reverse | CGCCGAGCTGGGAGTAAA |