Figure 4.

NRG1 Type III was decreased in sural nerve after 9 and 12 weeks of diabetes. Mice were treated with vehicle (Veh) or STZ and sural nerves were isolated at 9 (A) or 12 (B) weeks after the induction of diabetes (n = 6–7 per group). Protein lysates were prepared and NRG1 Type III level was determined by immunoblot using the two antibodies. (C): Bands were quantified, NRG1 Type III levels were normalized to β-actin and expressed as a fold of the levels in control sural nerves (*, p < 0.05 versus Veh; **, p < 0.01 versus Veh).