Table 3.

Statistics of unigenes/ESTs in DBM-DB and KONAGAbase

Data set		Total number	GC %	Size (Mb)	Mean (bp)	Max (bp)
DBM-DBa	Egg	70 234	45.3%	31.4	447	14 617
	Larva	69 008	47.9%	24.8	436	20 840
	Pupa	73 194	45.3%	32.5	444	20 840
	Adult	55 943	45.2%	30.1	443	14 617
	CRS	54 869	51.8%	24.9	454	20 804
	RFS	58 565	48.8%	27.4	468	20 840
	Total	171 262	43.0%	93.6	547	23 074
KONAGAbaseb	NCBI	1722	50.1%	1.1	645	16 113
	Midgut	12 406	49.2%	6.0	480	879
	Egg	6904	42.6%	3.1	446	855
	Testis	16 308	44.2%	9.7	446	880
	Larvae (4th)	147 370	42.8%	66.5	451	11 311
	Total	84 570	43.0%	47.8	564	16 249

^aSamples at four basic developmental stages of transcriptomes in DBM-DB are from DBM susceptible (Fuzhou-S) strain. CRF and RFS represent the chlorpyrifos- and fipronil-resistant strains, respectively. The total unigenes is the result of clustering and redundancy filtering of six transcriptomes.

^bKONAGAbase unigenes were assembled the EST/mRNA sequences from NCBI, the ESTs from midgut, egg and testes, and the RNA-seq contigs of the fourth instar DBM larvae.