A, VCAM-1 mRNA expression in cultured lung endothelial cells. Lung endothelial
cells were treated with or without 100nM PMA. VCAM-1 mRNA was determined by real
time PCR and normalized by 36B4 (ApoE-/- n=8, ApoE-/- +PMA,
n=3, Tg(Prkcb)ApoE-/- n=9, Tg(Prkcb)ApoE-/- + PMA
n=3). (B-C), VCAM-1 protein expression in cultured lung endothelial
cells. Lung endothelial cells were treated with or without 100nM insulin. VCAM-1
protein was determined by western blotting and normalized by actin. B indicates
representative western blots and C shows mean value of the ratio of VCAM-1 to
actin (n=4 for each group). D, Leukocyte-endothelial binding. The
binding of RAW 264.7 macrophages to monolayer lung endothelial cells from
ApoE-/- or Tg(Prkcb)ApoE-/- mice was measured (n=3 for each group).
(E-F), VCAM-1 protein expression in endothelial cells. Endothelial cells were
treated with or without 100nM insulin or 100nM PMA. VCAM-1 protein was
determined by western blotting and normalized by actin. E indicates
representative western blots and F shows mean value of the ratio of VCAM-1 to
actin (n=3 for each group).