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. Author manuscript; available in PMC: 2014 Feb 8.
Published in final edited form as: Nature. 2013 Jun 30;500(7461):222–226. doi: 10.1038/nature12362

Figure 3. The effects of Vitamin C are Tet-dependent.

Figure 3

a, Dose-dependent effect of VitC on in vitro Tet activity. n = 3 technical replicates, values are mean ± s.d. b, Dot blot for hmC following a 12 hr VitC treatment. c, hmC (top) and mC (bottom) DIP-qPCR following a 12 or 72 hr VitC treatment, respectively. d, Gene expression at 72 hrs of VitC treatment. qRT-PCR data expressed relative to untreated wild-type cells. For c, d, n = 2 biological replicates, values are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 by t-test throughout the figure.