Figure 2.

DTX with or without RS effects on the mRNA expression by M2 macrophages. M2 macrophages from PBMCs were stimulated by DTX and/or RS. Six hours after stimulation, total RNA was recovered from these cells and then reverse transcribed into cDNA. The viability of cells 6 hours after stimulation is 95%~98% in each group. Quantitative real-time PCR was conducted to determine the number of copies of cDNA for each chemokine, Arg1, and iNOS and the relative mRNA expression levels were calculated for each gene and each time point after normalization against GAPDH using the ΔΔCt method. The data from each donor were obtained by the triplicated assays, and then the mean ± SD was calculated. Representative data from three different donors are shown.