Figure 3. E47^hetp21^het LT-HSCs exhibit decreased self-renewal and persistence in vivo.

(A) Serial transplantation was performed by transferring 2 × 10⁶ BM cells from CD45.2⁺ WT, E47^het, p21^het or E47^hetp21^het mice into sub-lethally irradiated CD45.1⁺ C57BL/6 recipient mice to examine LT-HSC self-renewal and persistence through three rounds of serial transfer. (B) Mice were sacrificed at sixteen weeks post-transplant and the number of donor-derived BM LT-HSCs (CD45.2⁺ CD150⁺ CD48⁻ LSK or CD45.2⁺ CD150⁺ flk2⁻ LSK) was enumerated in primary, secondary or tertiary recipients. Graph is shown as mean ± SD of data pooled from n= 4–8 recipient mice for each genotype. (C) Mice were sacrificed two weeks after primary transplantation and the frequency of CD45.2⁺ total HSCs was examined. Data is shown as mean ± SD from n= 2–3 recipient mice per group. *p<0.05