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. Author manuscript; available in PMC: 2014 Jan 16.
Published in final edited form as: J Med Chem. 2010 Oct 14;53(19):7146–7155. doi: 10.1021/jm101144f

Table 7.

LanthaScreen™ profile of 26 in invitrogen lipid kinase panela

Kinase IC50(nM) Kinase IC50(nM)
PI4K α >10000 P110 α/p85α 250
PI4K β 6680 P110δ/P85α 564
PI3K-C2 α 176 P110γ 171
PI3K-C2β 549 DNA-PK 6.34
hVPS34 533 mTOR 4.32
a

Kinase targets were tested with biochemical enzymatic kinase assays using the SelectScreen® Kinase Profiling Service (Life Technologies Corporation, Madison, WI) to determine IC50 values as shown in Table 7. The compounds were assayed at 10 concentrations (three-fold serial dilutions starting from 1 μM) at an ATP concentration equal to the ATP Km,app for the assay following the detailed procedures described in the SelectScreen® Customer Protocol and Assay Conditions documents located at www.invitrogen.com/kinaseprofiling.