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. 2013 Dec 10;141(1):39–51. doi: 10.1111/imm.12164

Figure 5.

Figure 5

Mycobacterium tuberculosis infection results in a reduction of cell surface-expressed integrin heterodimers and individual α subunits. Dendritic cells (DCs) were cultured in medium alone or infected with M. tuberculosis (multiplicity of infection ∼ 2), harvested, and double-immunolabelled for either CD18/CD11a (LFA-1) or CD18/CD11b (Mac-1) and analysed by flow cytometry. Representative scatter plots of LFA-1 on medium alone cells (a) or M. tuberculosis-infected (b) are shown. Representative scatter plots of Mac-1 on medium alone cells (d) and M. tuberculosis-infected (e) are shown. Overlaid histogram representations of the same data sets highlight the changes in a CD11a (c) or CD11b (f) surface expression. (g) The mean fluorescent intensity (MFI) ± standard error for CD11a (αL) and CD11b (αM), with five combined experiments performed with independent donors, are shown. Representative histogram overlays for CD11a (c) and CD11b (f) are shown; grey = isotype control; thin line = infected; bold black line = medium alone.