Skip to main content
. 2013 Dec 10;141(1):61–69. doi: 10.1111/imm.12168

Figure 4.

Figure 4

Macrophage activation in the early stages after interferon-γ (IFN-γ) stimulation is reduced by the p38 mitogen-activated protein kinase (MAPK) inhibitors. RAW 264.7 cells or bone marrow-derived macrophages (BMMs) were treated with 200 U/ml IFN-γ in the absence or presence of p38 MAPK inhibitors (10 μm of PD 169316, 10 μm of SB202190, or 5 μm of SB 203580) for 4 hr. (a, b) The cells were then infected with Listeria monocytogenes (a) or Salmonella typhimurium (b), and bacterial growth in the cells was calculated on the basis of colony-forming units (CFU). The values shown are the mean ± standard deviation values obtained from three independent experiments. *P < 0·01; **P < 0·05; ***P < 0·1. (c) The cells were lysed in cell lysis buffer containing protease inhibitors, and the cell lysates were then subjected to SDS–PAGE analysis and Western blotting. Densitometric LC3-II/GAPDH ratios are shown underneath the blot.