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Equlibrium Microdialysis. The binding of napyradiomycins CNQ525.510B (1) or A80915C (2) with recombinant hGrp94 was determined by equilibrium microdialysis. LC-MS analysis using UV-Vis detection at 380 nm and peaks were validated by MS analysis. The LC-MS method was standardized with known concentrations prior to use. Three repetitions were conducted at each concentration and the deviation between these experiments depicted by error bars. Experiments were conducted with 20 μM hGrp94.
