Table 1.
List of All the Tryptic Peptides of Human eNOS Detected Using Mass Spectrometry Which Contained NO-Reactive Cysteine Residues
| Peptides detected | Position in eNOS sequence | Nitrosylated cysteine position | Sequence consensus motif (−2, −1, 0, 1, 2), with 0 being cysteine | Techniques used |
|---|---|---|---|---|
| 1048.1 Da, +1 | 98–106 | 98 | RRCLG | LC-MSa |
| 597.1 Da, +2 | 1102–1107 | 1104 | VLCLE | nLC-MS/MS |
| 814.4 Da, +2 | 846–855 | 852 | PPCTL | nLC-MS/MS |
| 945.8 Da, +2 | 225–237 | 234 | QRCPG | nLC-MS/MS |
| 1597.5 Da, +1 | 846–855 | 852 | PPCTL | MALDI-MS |
| 2300.2 Da, +1 | 221–237 | 234 | QRCPG | MALDI-MS |
| 3352.7 Da, +1 | 849–874 | 852 | PPCTL | MALDI-MS |
| 3798.9 Da, +1 | 05–35 | 14/25 | PPCGL/GLCGK | MALDI-MS |
| 3813.2 Da, +1 | 963–993 | 975 or 990 | GVCST or VPCFI | MALDI-MS |
| 2218.8 Da, +1 | 1000–1021 | 1008 | LPCIL | Neutravidin, GSNO, MALDI-MS |
| 2964.5 Da, +1 | 70–96 | 93 | GPCTP | Neutravidin, GSNO, MALDI-MS |
| 4244.5 Da, +1 | 776–815 | 801 | GVCPP | Neutravidin, GSNO, MALDI-MS |
| 3122.2 Da, +1 | 70–97 | 93 | GPCTP | Neutravidin, SNAP, MALDI-MS |
| 5234.3 Da, +1 | 1102–1149 | 1104 or 1113 | VLCLE or FVCGD | Neutravidin, SNAP, MALDI-MS |
a
Lu et al. (2005).