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Involvement of ADH in neurosteroidogenesis. A, Immunostaining against 5α-reduced neurosteroids in the CA1 area (left panel) is enhanced by 60 mM ethanol (second panel). 4MP (1 mM), an ADH inhibitor, blocks the effects of ethanol (third) but does not alter basal staining (fourth panel). Calibration bar: 25 μm. B, Summary of immunohistological studies shows fluorescence intensity as mean ± s.e.m (n = 5). P value was calculated by Holm-Sidak post-hoc method. * P < 0.001.
