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Effects of a catalase inhibitor on neurosteroidogenesis. A, Immunostaining against 5α-reduced neurosteroids in the CA1 area (left panel) is enhanced by 60 mM ethanol (second). Sodium azide has no effect on ethanol (third) and does not alter basal staining (fourth panel). Calibration bar: 25 μm. B, Summary of immunohistological studies with sodium azide shows fluorescence intensity as mean ± s.e.m (n = 5). P value was calculated by Holm-Sidak post-hoc method. * P < 0.001.
