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Effects of a CYP2E1 inhibitor on neurosteroidogenesis. A, Immunostaining against 5α-reduced neurosteroids in the CA1 area (left) is enhanced by 60 mM ethanol (second panel) and ethanol’s effects are not blocked by allyl sulfide, a CYP2E1 inhibitor (third panel). Allyl sulfide alone had no effect on basal staining (fourth panel). Calibration bar: 25 μm. B, Summary of immunohistological studies with allyl sulfide shows fluorescence intensity as mean ± s.e.m.
