Table 2.
Effect of PEITC-NAC plus MI on the multiplicity of NNK plus BaP-induced microscopic lung lesionsa
| Group | Temporal sequence | Compound (dose, μmol/g diet) | Carcinogen (dose, μmol) | No. of mice used for the analysis | Hperplastic foci/mouse | Reduction (%) | Adenoma/mouse | Reduction (%) | Adenoma with cellular pleomorphism/mouse | Reduction (%) | Adeno-carcinoma/mouse | Reduction (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NNK (2) +BaP (2) | 19 | 2.22 ± 1.07 | 1.07 ± 0.70 | 3.94 ± 1.68 | 0.86 ± 0.59 | ||||||
| 2 | NNK (1) +BaP (1) | 18 | 0.87 ± 0.38 | 0.20 ± 0.19 | 1.31 ± 0.44 | 0.24 ± 0.15 | ||||||
| 3 | A | PEITC-NAC (15) +MI (56 | NNK (2) +BaP (2) | 14 | 1.42 ± 0.52 | 36%** | 0.71 ± 0.32 | 35% | 0.90 ± 0.81 | 77%** | 0.23 ± 0.34 | 73%** |
| 4 | A | PEITC-NAC (15) +MI (56 | NNK (1) +BaP (1) | 17 | 0.49 ± 0.52 | 42%* | 0.16 ± 0.19 | 20% | 0.39 ± 0.30 | 70%* | 0.18 ± 0.19 | 25% |
| 5 | A | PEITC-NAC (9) +MI (56) | NNK (2) +BaP (2) | 22 | 1.23 ± 0.57 | 45%** | 0.61 ± 0.36 | 42% | 0.98 ± 0.51 | 75%** | 0.31 ± 0.24 | 64%** |
| 6 | A | PEITC-NAC (9) +MI (56) | NNK (1) +BaP (1) | 21 | 0.46 ± 0.21 | 47%* | 0.24 ± 0.10 | none | 0.30 ± 0.11 | 77%* | 0.06 ± 0.11 | 75%* |
| 7 | B | PEITC-NAC (15) +MI (56) | NNK (2) +BaP (2) | 25 | 2.56 ± 1.05 | none | 1.27 ± 0.92 | none | 1.64 ± 0.87 | 58%** | 0.40 ± 0.41 | 53%** |
| 8 | – | PEITC-NAC (15) +MI (56) | none | 10 | 0.25 ± 0.50 | – | – | – | ||||
| 9 | – | PEITC-NAC (9) +MI (56) | none | 10 | 0.13 ± 0.25 | – | – | – | ||||
| 10 | none | none | 5 | 0.33 ± 0.55 | 0.33 ± 0.55 | – | – |
a
Groups of female A/J mice received by gavage eight weekly doses of a mixture of NNK plus BaP in 0.1 ml cottonseed oil. The mice were maintained on AIN-93G diet from age 5–6 weeks until 1 week after the end of carcinogen treatment and then shifted to AIN-93M diet for the duration of the experiment. PEITC-NAC + MI was added to the diet in temporal sequence A or B.
*
P < 0.05, compared to group 2.
**
P < 0.05, compared to group 1.