Cqu comm2a (A, B), Cqu comm2b (C, D), and Cqucomm2c (E, F) transcripts are detected in the developing C. quinquefasciatus nerve cord and head region (both brain and overlying tissue). Arrows mark nerve cord expression on the ventral sides of 22 hour old embryos; lateral views oriented anterior left and ventral down are shown. Ventral views of CNS expression in these same animals (B, D, F; anterior is again oriented left) are shown to the right of panels A, C, and E, respectively. All three genes are widely expressed in the developing nerve cord during the period of commissural axon guidance, but the neural expression levels of Cqu comm2b (F) are markedly less than the neural expression levels of the other two comm2 genes (B, D).