Figure 3. SP retention in mated females, 4 days after mating.

Western blots probed with antibodies to SP or alpha-tubulin (loading control). Proteins were isolated from lower female reproductive tracts 4 days after mating. Gene names to the left of each pair of blots indicate which gene was (KD) or was not (cont) knocked down in the mating pair. Across all experiments, the number of female reproductive tract (RT) equivalents used for each condition ranged from 13 to 20; however, for any given gene, the number of RT equivalents compared between KD and control was within 2 RTs.